Review



cell culture 852 human embryonic kidney hek 293ft cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC cell culture 852 human embryonic kidney hek 293ft cells
    Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm42015511-356-2-11
    Average 99 stars, based on 38021 article reviews
    cell culture 852 human embryonic kidney hek 293ft cells - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Real-time Polymerase Chain Reaction:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Luciferase:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Reporter Gene Assay:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Transfection:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Article Title: Genetic polymorphisms in SLCO2B1 and ABCC1 conjointly modulate atorvastatin intracellular accumulation in HEK293 recombinant cell lines
    Article Snippet: Gentamicin (G418), hygromycin B, dimethyl sulfoxide (DMSO), ampicillin and kanamycin were purchased from Sigma-Aldrich (St. Louis, MO). .. Cell culture Human Embryonic Kidney cells (HEK 293, ATCC N°CRL-1573) control and transfected cells were grown in Dulbecco’s Modi ed Eagle Medium with high glucose, L-glutamine and sodium pyruvate (DMEM GIBCO®) supplemented with 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) of penicillin-streptomycin purchased from ThermoFisher Scienti c (Waltham, MA). ..

    Quantitative RT-PCR:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Software:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Cell Culture:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Article Title: A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation
    Article Snippet: .. Cell Culture Human embryonic kidney cells (HEK293) were purchased from ATCC (CRL-1573) and were grown at 37°C under 5% CO 2 , in a Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10% L-glutamine, and antibiotics (100 IU mL−1 penicillin and 100 mg mL –1 streptomycin) (University of California, San Francisco Cell Culture Facility). ..

    Article Title: Genetic polymorphisms in SLCO2B1 and ABCC1 conjointly modulate atorvastatin intracellular accumulation in HEK293 recombinant cell lines
    Article Snippet: Gentamicin (G418), hygromycin B, dimethyl sulfoxide (DMSO), ampicillin and kanamycin were purchased from Sigma-Aldrich (St. Louis, MO). .. Cell culture Human Embryonic Kidney cells (HEK 293, ATCC N°CRL-1573) control and transfected cells were grown in Dulbecco’s Modi ed Eagle Medium with high glucose, L-glutamine and sodium pyruvate (DMEM GIBCO®) supplemented with 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) of penicillin-streptomycin purchased from ThermoFisher Scienti c (Waltham, MA). ..

    Article Title: HAND2 Assists MYCN Enhancer Invasion to Regulate a Noradrenergic Neuroblastoma Phenotype
    Article Snippet: .. MATERIALS AND METHODS Cell culture Human embryonic kidney cells (HEK293T) and Lenti-X HEK293 were obtained from ATCC and were maintained in Dulbecco’s modified Eagle’s media. ..

    Article Title: Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis
    Article Snippet: .. Cell culture Human embryonic kidney cells (HEK293T, American Type Culture Collection (ATCC), Manassas, VA, USA), cultured in a T75 cell flask with media, were incubated at 37 ◦C and 5% CO2. .. Media comprised of 90 v/v% Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, Waltham, MA, USA), 10 v/v% fetal bovine serum (FBS, ThermoFisher Scientific), and 2 mM each of glutamax (ThermoFisher Scientific) and 2% penicillin-streptomycin (ThermoFisher Scientific).

    Article Title: Synthesis and antiproliferative activities of doxorubicin thiol conjugates and doxorubicin-SS-cyclic peptide
    Article Snippet: .. Cell culture Human embryonic kidney cells (HEK-293, ATCC No. CRL-1573), human ovarian cancer cells (SKOV-3, ATCC No. HTB-77), human fibrosarcoma cells (HT-1080, ATCC No. CRL-12012), human leukemia cell line (CCRF-CEM, ATCC No. CCL-119), human breast adenocarcinoma (MDA-MB-468, ATCC No. HTB-132), human breast adenocarcinoma cell (MCF-7, ATCC No. HTB-22), and mouse myoblast cells (C2C12, ATCC No. CRL-1772) were purchased from American Type Culture Collection. ..

    Article Title: Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconate.
    Article Snippet: Synthetic gene fragments used in the study were codon-optimized for expression in human cells and synthesized by Twist Bioscience. .. Cell culture Human embryonic kidney cells (HEK-293T, ATCC: CRL11268) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; 10566016, Thermo Fischer) supplemented with 10% v / v fetal bovine serum (FBS; F7524, Sigma Aldrich) under a humidified atmosphere containing 5% CO 2 at 37 ◦C. ..

    Modification:

    Article Title: A digital CRISPR-dCas9-based gene remodeling biocomputer programmed by dietary compounds in mammals.
    Article Snippet: In brief Yin et al. develop a dual-input programmed CRISPR-dCas9-based gene modulation biocomputer (REPACRISPR) for conditional endogenous gene transcriptional regulation and mathematical models to predict doseresponsive endogenous gene transcription.. This work expands the field of controllable epigenetic remodeling and provides a logic control method for gene function research and gene therapy.

    Article Title: A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation
    Article Snippet: .. Cell Culture Human embryonic kidney cells (HEK293) were purchased from ATCC (CRL-1573) and were grown at 37°C under 5% CO 2 , in a Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10% L-glutamine, and antibiotics (100 IU mL−1 penicillin and 100 mg mL –1 streptomycin) (University of California, San Francisco Cell Culture Facility). ..

    Article Title: HAND2 Assists MYCN Enhancer Invasion to Regulate a Noradrenergic Neuroblastoma Phenotype
    Article Snippet: .. MATERIALS AND METHODS Cell culture Human embryonic kidney cells (HEK293T) and Lenti-X HEK293 were obtained from ATCC and were maintained in Dulbecco’s modified Eagle’s media. ..

    Article Title: Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconate.
    Article Snippet: Synthetic gene fragments used in the study were codon-optimized for expression in human cells and synthesized by Twist Bioscience. .. Cell culture Human embryonic kidney cells (HEK-293T, ATCC: CRL11268) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; 10566016, Thermo Fischer) supplemented with 10% v / v fetal bovine serum (FBS; F7524, Sigma Aldrich) under a humidified atmosphere containing 5% CO 2 at 37 ◦C. ..

    Control:

    Article Title: Genetic polymorphisms in SLCO2B1 and ABCC1 conjointly modulate atorvastatin intracellular accumulation in HEK293 recombinant cell lines
    Article Snippet: Gentamicin (G418), hygromycin B, dimethyl sulfoxide (DMSO), ampicillin and kanamycin were purchased from Sigma-Aldrich (St. Louis, MO). .. Cell culture Human Embryonic Kidney cells (HEK 293, ATCC N°CRL-1573) control and transfected cells were grown in Dulbecco’s Modi ed Eagle Medium with high glucose, L-glutamine and sodium pyruvate (DMEM GIBCO®) supplemented with 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) of penicillin-streptomycin purchased from ThermoFisher Scienti c (Waltham, MA). ..

    Incubation:

    Article Title: Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis
    Article Snippet: .. Cell culture Human embryonic kidney cells (HEK293T, American Type Culture Collection (ATCC), Manassas, VA, USA), cultured in a T75 cell flask with media, were incubated at 37 ◦C and 5% CO2. .. Media comprised of 90 v/v% Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, Waltham, MA, USA), 10 v/v% fetal bovine serum (FBS, ThermoFisher Scientific), and 2 mM each of glutamax (ThermoFisher Scientific) and 2% penicillin-streptomycin (ThermoFisher Scientific).

    Multiple Displacement Amplification:

    Article Title: Synthesis and antiproliferative activities of doxorubicin thiol conjugates and doxorubicin-SS-cyclic peptide
    Article Snippet: .. Cell culture Human embryonic kidney cells (HEK-293, ATCC No. CRL-1573), human ovarian cancer cells (SKOV-3, ATCC No. HTB-77), human fibrosarcoma cells (HT-1080, ATCC No. CRL-12012), human leukemia cell line (CCRF-CEM, ATCC No. CCL-119), human breast adenocarcinoma (MDA-MB-468, ATCC No. HTB-132), human breast adenocarcinoma cell (MCF-7, ATCC No. HTB-22), and mouse myoblast cells (C2C12, ATCC No. CRL-1772) were purchased from American Type Culture Collection. ..



    Similar Products

    cell culture 852 human embryonic kidney hek 293ft cells  (ATCC)
    99
    ATCC cell culture 852 human embryonic kidney hek 293ft cells
    Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm42015511-356-2-11
    Average 99 stars, based on 1 article reviews
    cell culture 852 human embryonic kidney hek 293ft cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    press cell culture human embryonic kidney cells  (ATCC)
    99
    ATCC press cell culture human embryonic kidney cells
    Press Cell Culture Human Embryonic Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm41888545-57-9-17
    Average 99 stars, based on 1 article reviews
    press cell culture human embryonic kidney cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human embryonic kidney 293t hek293t cells cells american type culture collection  (ATCC)
    99
    ATCC human embryonic kidney 293t hek293t cells cells american type culture collection
    a, Fluorescent in situ hybridization against GFP for clonal barcode transcripts in <t>HEK293T</t> cells. Top, cells infected with original LARRY barcode from Weinreb et al. Bottom, cells infected with LARRY barcode modified by addition of mU1 hairpin sequence. Scale bar 50 μm. Representative of n = 100 cells across 2 independent experiments. b, Schematic detailing molecular process of capturing clonal barcodes and computational workflow for assigning cells to clones. c, HNF4/PPAR motif accessibility amongst the 50 largest clones by cell number. Each violin plot represents distribution of motif scores for an individual clone and point represents median values per clone. d, Framework for computing clonal variance and identifying clonal features. e, Motif accessibility relative to position in the colon. The X-axis represents segment of the colon, with segment 1 being most proximal and segment 6 being most distal. The Y-axis shows change in motif accessibility of a given segment relative to segment 1. f, Accessibility of all TF motif families relative to position in the colon. Each row represents a TF motif family and color indicates change in motif accessibility relative to segment 1. g, Expression stemness ( Lgr5 , Lrig1 ) and differentiation ( Car1 ) markers in relation to HNF4/PPAR motif score. h, Downsampling analysis of permutation-based clonal heritability. Clones were randomly sampled to the number indicated on the x-axis and permutation-based testing was performed identically to the complete dataset. The total number of motif families identified to have lower clonal variance as compared to random (FDR < 0.05) for each clone number is shown on the y-axis. i, Linear mixed model of random effects from exposure to colitis and clonal identity. j, Variance explained by clonal identity from the linear mixed model. k, Comparison of variance explained by clonal identity and exposure to colitis for all TF motif families. l, Left, variance explained by clonal identity and exposure to colitis for top TF motif families. Right, variance attributed to each factor following randomization of clonal identities within colitis or control conditions. m, Comparison of clonality identified by permutation and linear mixed model. The X-axis shows -log10(FDR) from the permutation method (Fig. ) and the Y-axis shows percent variance attributed to clonal identity in the linear mixed model (panel j). Panels created in BioRender: e , Nagaraja, S. https://biorender.com/jqhj3wt (2026); i , Nagaraja, S. https://biorender.com/4mut8sd (2026).
    Human Embryonic Kidney 293t Hek293t Cells Cells American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pmc13083248-211-0-6
    Average 99 stars, based on 1 article reviews
    human embryonic kidney 293t hek293t cells cells american type culture collection - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    cell lines human embryonic kidney 293t hek293t american type culture collection  (ATCC)
    99
    ATCC cell lines human embryonic kidney 293t hek293t american type culture collection
    a, Fluorescent in situ hybridization against GFP for clonal barcode transcripts in <t>HEK293T</t> cells. Top, cells infected with original LARRY barcode from Weinreb et al. Bottom, cells infected with LARRY barcode modified by addition of mU1 hairpin sequence. Scale bar 50 μm. Representative of n = 100 cells across 2 independent experiments. b, Schematic detailing molecular process of capturing clonal barcodes and computational workflow for assigning cells to clones. c, HNF4/PPAR motif accessibility amongst the 50 largest clones by cell number. Each violin plot represents distribution of motif scores for an individual clone and point represents median values per clone. d, Framework for computing clonal variance and identifying clonal features. e, Motif accessibility relative to position in the colon. The X-axis represents segment of the colon, with segment 1 being most proximal and segment 6 being most distal. The Y-axis shows change in motif accessibility of a given segment relative to segment 1. f, Accessibility of all TF motif families relative to position in the colon. Each row represents a TF motif family and color indicates change in motif accessibility relative to segment 1. g, Expression stemness ( Lgr5 , Lrig1 ) and differentiation ( Car1 ) markers in relation to HNF4/PPAR motif score. h, Downsampling analysis of permutation-based clonal heritability. Clones were randomly sampled to the number indicated on the x-axis and permutation-based testing was performed identically to the complete dataset. The total number of motif families identified to have lower clonal variance as compared to random (FDR < 0.05) for each clone number is shown on the y-axis. i, Linear mixed model of random effects from exposure to colitis and clonal identity. j, Variance explained by clonal identity from the linear mixed model. k, Comparison of variance explained by clonal identity and exposure to colitis for all TF motif families. l, Left, variance explained by clonal identity and exposure to colitis for top TF motif families. Right, variance attributed to each factor following randomization of clonal identities within colitis or control conditions. m, Comparison of clonality identified by permutation and linear mixed model. The X-axis shows -log10(FDR) from the permutation method (Fig. ) and the Y-axis shows percent variance attributed to clonal identity in the linear mixed model (panel j). Panels created in BioRender: e , Nagaraja, S. https://biorender.com/jqhj3wt (2026); i , Nagaraja, S. https://biorender.com/4mut8sd (2026).
    Cell Lines Human Embryonic Kidney 293t Hek293t American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm41709459-589-86-93
    Average 99 stars, based on 1 article reviews
    cell lines human embryonic kidney 293t hek293t american type culture collection - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    culture human embryonic kidney hek 293t cells  (ATCC)
    99
    ATCC culture human embryonic kidney hek 293t cells
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
    Culture Human Embryonic Kidney Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T%2F17/pmc12911911-160-17-24
    Average 99 stars, based on 1 article reviews
    culture human embryonic kidney hek 293t cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    cell culture human embryonic kidney 293t  (ATCC)
    99
    ATCC cell culture human embryonic kidney 293t
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
    Cell Culture Human Embryonic Kidney 293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm41611810-300-0-8
    Average 99 stars, based on 1 article reviews
    cell culture human embryonic kidney 293t - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    cell culture human embryonic kidney cells 293 ft  (ATCC)
    96
    ATCC cell culture human embryonic kidney cells 293 ft
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
    Cell Culture Human Embryonic Kidney Cells 293 Ft, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/Human+embryonic+kidney+cells+293SF-3F6/pm41537969-95-0-14
    Average 96 stars, based on 1 article reviews
    cell culture human embryonic kidney cells 293 ft - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    cell culture conditions human hek293t embryonic kidney cells  (ATCC)
    99
    ATCC cell culture conditions human hek293t embryonic kidney cells
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
    Cell Culture Conditions Human Hek293t Embryonic Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+cells/293T/pm41519940-213-4-18
    Average 99 stars, based on 1 article reviews
    cell culture conditions human hek293t embryonic kidney cells - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    a, Fluorescent in situ hybridization against GFP for clonal barcode transcripts in HEK293T cells. Top, cells infected with original LARRY barcode from Weinreb et al. Bottom, cells infected with LARRY barcode modified by addition of mU1 hairpin sequence. Scale bar 50 μm. Representative of n = 100 cells across 2 independent experiments. b, Schematic detailing molecular process of capturing clonal barcodes and computational workflow for assigning cells to clones. c, HNF4/PPAR motif accessibility amongst the 50 largest clones by cell number. Each violin plot represents distribution of motif scores for an individual clone and point represents median values per clone. d, Framework for computing clonal variance and identifying clonal features. e, Motif accessibility relative to position in the colon. The X-axis represents segment of the colon, with segment 1 being most proximal and segment 6 being most distal. The Y-axis shows change in motif accessibility of a given segment relative to segment 1. f, Accessibility of all TF motif families relative to position in the colon. Each row represents a TF motif family and color indicates change in motif accessibility relative to segment 1. g, Expression stemness ( Lgr5 , Lrig1 ) and differentiation ( Car1 ) markers in relation to HNF4/PPAR motif score. h, Downsampling analysis of permutation-based clonal heritability. Clones were randomly sampled to the number indicated on the x-axis and permutation-based testing was performed identically to the complete dataset. The total number of motif families identified to have lower clonal variance as compared to random (FDR < 0.05) for each clone number is shown on the y-axis. i, Linear mixed model of random effects from exposure to colitis and clonal identity. j, Variance explained by clonal identity from the linear mixed model. k, Comparison of variance explained by clonal identity and exposure to colitis for all TF motif families. l, Left, variance explained by clonal identity and exposure to colitis for top TF motif families. Right, variance attributed to each factor following randomization of clonal identities within colitis or control conditions. m, Comparison of clonality identified by permutation and linear mixed model. The X-axis shows -log10(FDR) from the permutation method (Fig. ) and the Y-axis shows percent variance attributed to clonal identity in the linear mixed model (panel j). Panels created in BioRender: e , Nagaraja, S. https://biorender.com/jqhj3wt (2026); i , Nagaraja, S. https://biorender.com/4mut8sd (2026).

    Journal: Nature

    Article Title: Epigenetic memory of colitis promotes tumour growth

    doi: 10.1038/s41586-026-10258-4

    Figure Lengend Snippet: a, Fluorescent in situ hybridization against GFP for clonal barcode transcripts in HEK293T cells. Top, cells infected with original LARRY barcode from Weinreb et al. Bottom, cells infected with LARRY barcode modified by addition of mU1 hairpin sequence. Scale bar 50 μm. Representative of n = 100 cells across 2 independent experiments. b, Schematic detailing molecular process of capturing clonal barcodes and computational workflow for assigning cells to clones. c, HNF4/PPAR motif accessibility amongst the 50 largest clones by cell number. Each violin plot represents distribution of motif scores for an individual clone and point represents median values per clone. d, Framework for computing clonal variance and identifying clonal features. e, Motif accessibility relative to position in the colon. The X-axis represents segment of the colon, with segment 1 being most proximal and segment 6 being most distal. The Y-axis shows change in motif accessibility of a given segment relative to segment 1. f, Accessibility of all TF motif families relative to position in the colon. Each row represents a TF motif family and color indicates change in motif accessibility relative to segment 1. g, Expression stemness ( Lgr5 , Lrig1 ) and differentiation ( Car1 ) markers in relation to HNF4/PPAR motif score. h, Downsampling analysis of permutation-based clonal heritability. Clones were randomly sampled to the number indicated on the x-axis and permutation-based testing was performed identically to the complete dataset. The total number of motif families identified to have lower clonal variance as compared to random (FDR < 0.05) for each clone number is shown on the y-axis. i, Linear mixed model of random effects from exposure to colitis and clonal identity. j, Variance explained by clonal identity from the linear mixed model. k, Comparison of variance explained by clonal identity and exposure to colitis for all TF motif families. l, Left, variance explained by clonal identity and exposure to colitis for top TF motif families. Right, variance attributed to each factor following randomization of clonal identities within colitis or control conditions. m, Comparison of clonality identified by permutation and linear mixed model. The X-axis shows -log10(FDR) from the permutation method (Fig. ) and the Y-axis shows percent variance attributed to clonal identity in the linear mixed model (panel j). Panels created in BioRender: e , Nagaraja, S. https://biorender.com/jqhj3wt (2026); i , Nagaraja, S. https://biorender.com/4mut8sd (2026).

    Article Snippet: Human embryonic kidney 293T (HEK293T) cells (American Type Culture Collection (ATCC), CRL-3216; authenticated by short tandem repeat profiling and tested for mycoplasma by ATCC) were grown in DMEM (Thermo, 11965-092) with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: In Situ Hybridization, Infection, Modification, Sequencing, Clone Assay, Expressing, Comparison, Control

    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) HEK 293T cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) HEK 293T cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Transfection, Co-Immunoprecipitation Assay, Expressing, Pull Down Assay, Labeling, Immunofluorescence, Microscopy, Western Blot, Plasmid Preparation

    ALDH1L1 inhibits PEDV replication via the E3 ubiquitin ligase STUB1. ( A ) After HEK 293T cells were cotransfected with plasmids encoding HA-STUB1 and Flag-ALDH1L1, the Co-IP assay was performed using anti-Flag-bound beads. ( B ) After HEK 293T cells were transfected with plasmids coding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-STUB1 and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and STUB1 was examined via the GST pull-down assay. ( D ) After cotransfecting HeLa cells with Flag-ALDH1L1 and STUB1-HA for 24 h via incubation with an anti-Flag Mab, ALDH1L1 and STUB1 colocalization was confirmed via confocal immunofluorescence microscopy. Scale bars = 100 µm. ( E and F ) After cotransfection with Myc-ALDH1L1, HA-Ub, HA-STUB1, and Flag-N, or Flag-E, HEK 293T cells were treated with CQ and collected. The ubiquitinated N and E proteins were subjected to western blotting after immunoprecipitation with an anti-Flag antibody. ( G and H ) After cotransfecting HEK 293T cells with siRNAs (negative control or STUBA siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed with an anti-HA antibody.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 inhibits PEDV replication via the E3 ubiquitin ligase STUB1. ( A ) After HEK 293T cells were cotransfected with plasmids encoding HA-STUB1 and Flag-ALDH1L1, the Co-IP assay was performed using anti-Flag-bound beads. ( B ) After HEK 293T cells were transfected with plasmids coding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-STUB1 and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and STUB1 was examined via the GST pull-down assay. ( D ) After cotransfecting HeLa cells with Flag-ALDH1L1 and STUB1-HA for 24 h via incubation with an anti-Flag Mab, ALDH1L1 and STUB1 colocalization was confirmed via confocal immunofluorescence microscopy. Scale bars = 100 µm. ( E and F ) After cotransfection with Myc-ALDH1L1, HA-Ub, HA-STUB1, and Flag-N, or Flag-E, HEK 293T cells were treated with CQ and collected. The ubiquitinated N and E proteins were subjected to western blotting after immunoprecipitation with an anti-Flag antibody. ( G and H ) After cotransfecting HEK 293T cells with siRNAs (negative control or STUBA siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed with an anti-HA antibody.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Ubiquitin Proteomics, Co-Immunoprecipitation Assay, Transfection, Expressing, Pull Down Assay, Incubation, Immunofluorescence, Microscopy, Cotransfection, Western Blot, Immunoprecipitation, Negative Control

    ALDH1L1 suppresses PEDV replication via the cargo receptor TOLLIP. ( A ) After cotransfecting HEK 293T cells with plasmids coding HA-TOLLIP and Flag-ALDH1L1, a Co-IP assay was performed with anti-Flag-bound beads. ( B ) After transfecting HEK 293T cells with plasmids encoding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-TOLLIP and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and TOLLIP was examined via the GST pull-down assay. ( D ) HeLa cells were transfected with plasmids coding Flag-ALDH1L1 and HA-TOLLIP. Then, the cells were incubated with specific antibodies and visualized under a confocal immunofluorescence microscope. Scale: 100 µm. ( E and F ) After cotransfecting HEK 293T cells with siRNAs (negative control or TOLLIP siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed using an anti-HA antibody.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 suppresses PEDV replication via the cargo receptor TOLLIP. ( A ) After cotransfecting HEK 293T cells with plasmids coding HA-TOLLIP and Flag-ALDH1L1, a Co-IP assay was performed with anti-Flag-bound beads. ( B ) After transfecting HEK 293T cells with plasmids encoding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-TOLLIP and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and TOLLIP was examined via the GST pull-down assay. ( D ) HeLa cells were transfected with plasmids coding Flag-ALDH1L1 and HA-TOLLIP. Then, the cells were incubated with specific antibodies and visualized under a confocal immunofluorescence microscope. Scale: 100 µm. ( E and F ) After cotransfecting HEK 293T cells with siRNAs (negative control or TOLLIP siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed using an anti-HA antibody.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Co-Immunoprecipitation Assay, Expressing, Pull Down Assay, Transfection, Incubation, Immunofluorescence, Microscopy, Negative Control, Western Blot